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anti human tim 3 mouse monoclonal antibody  (R&D Systems)


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    Structured Review

    R&D Systems anti human tim 3 mouse monoclonal antibody
    Anti Human Tim 3 Mouse Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+tim+3+pe/Human+TIM-4+PE-conjugated+Antibody/us12391757-36-19-28
    Average 93 stars, based on 1 article reviews
    anti human tim 3 mouse monoclonal antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Labeling:

    Article Title: Identification of CCL1 as a Gene Differentially Expressed in CD4 + T Cells Expressing TIM-3
    Article Snippet: .. For TIM-3 detection, cells were labeled with anti-human TIM-3-PE (R&D Systems, Minneapolis, MN, USA) and analyzed using a FACSCanto II apparatus (Becton Dickinson, San Diego, CA, USA). .. Total RNAs of T5 and G2 were extracted using a RNA isolation kit RNAiso (TaKaRa Bio, Shiga, Japan). cDNA synthesis and RT-PCR were performed following the procedures recommended by the manufacturer using an annealing control primer system (Seegene, Seoul, Korea).

    Control:

    Article Title: Down-Regulation of Tim-3 in Monocytes and Macrophages in Plasmodium Infection and Its Association with Parasite Clearance
    Article Snippet: .. The antibodies used in this study were as follows: Anti-Human CD14 PerCP-Cy5.5, mouse IgG1κ isotype control PerCP-Cy5.5, anti-mouse CD11b FITC, rat IgG2a K isotype control FITC, anti-mouse TIM-3 PE, and rat IgG2α K isotype control PE (all obtained from eBioscience, San Diego, CA, USA), anti-human Tim-3-PE, and rat IgG 2A isotope control-PE (both obtained from R&D Systems). .. The cells were detected and analyzed using a FACS Canto II flow cytometer (BD Biosciences, San Jose, CA, USA), and the gates for positive cells were defined using the isotype controls.

    Article Title: High Interleukin-37 (IL-37) Expression and Increased Mucin-Domain Containing-3 (TIM-3) on Peripheral T Cells in Patients with Rheumatoid Arthritis
    Article Snippet: .. Peripheral blood mononuclear cells was stained using the following fluorochrome-conjugated monoclonal antibodies: anti-human CD3 APC-eFluor780, anti-human CD8 FITC, anti-human CD127 PE-Cyanine7, anti-human CD14 PerCP− Cyanine 5.5 or isotype matched controls (all from eBioscience, San Diego, CA, USA); anti-human CD4 Alexa Fluor 700, anti-human CD25 PE/Dazzle 594 or isotype matched control (all from BioLegend, San Diego, CA, USA); and anti-human Tim-3 PE or isotype matched control (R&D, Minneapolis, MN, USA). .. Data were obtained from multicolor analysis using a FACS Calibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) and were analyzed using FlowJo software 7.6.2 (Tree Star, Ashland, OR, USA).

    Article Title: T-Cell Immunoglobulin- and Mucin-Domain-Containing Molecule 3 Signaling Blockade Improves Cell-Mediated Immunity Against Malaria.
    Article Snippet: .. The antibodies used in this study were as follows: anti-human CD3 PerCP-Cy5.5, anti-human CD4 FITC, antihuman CD8a APC, anti-human CD16/56 FITC, mouse IgG1κ isotype control PerCP-Cy5.5, mouse IgG1κ isotype control FITC, mouse IgG1κ isotype control APC, anti-mouse CD3e PE-Cy5, anti-mouse CD4 FITC, anti-mouse CD8a FITC, antimouse NK1.1 FITC, anti-mouse TIM-3 PE, rat IgG2a K isotype control PE-Cy5, rat IgG2a K isotype control FITC, and rat IgG2α K isotype control PE (all obtained from eBioscience, San Diego, California); and anti-human Tim-3-PE and rat IgG2A isotope control-PE (both obtained from R&D Systems, Minneapolis, MN). ..

    Staining:

    Article Title: High Interleukin-37 (IL-37) Expression and Increased Mucin-Domain Containing-3 (TIM-3) on Peripheral T Cells in Patients with Rheumatoid Arthritis
    Article Snippet: .. Peripheral blood mononuclear cells was stained using the following fluorochrome-conjugated monoclonal antibodies: anti-human CD3 APC-eFluor780, anti-human CD8 FITC, anti-human CD127 PE-Cyanine7, anti-human CD14 PerCP− Cyanine 5.5 or isotype matched controls (all from eBioscience, San Diego, CA, USA); anti-human CD4 Alexa Fluor 700, anti-human CD25 PE/Dazzle 594 or isotype matched control (all from BioLegend, San Diego, CA, USA); and anti-human Tim-3 PE or isotype matched control (R&D, Minneapolis, MN, USA). .. Data were obtained from multicolor analysis using a FACS Calibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) and were analyzed using FlowJo software 7.6.2 (Tree Star, Ashland, OR, USA).

    Bioprocessing:

    Article Title: High Interleukin-37 (IL-37) Expression and Increased Mucin-Domain Containing-3 (TIM-3) on Peripheral T Cells in Patients with Rheumatoid Arthritis
    Article Snippet: .. Peripheral blood mononuclear cells was stained using the following fluorochrome-conjugated monoclonal antibodies: anti-human CD3 APC-eFluor780, anti-human CD8 FITC, anti-human CD127 PE-Cyanine7, anti-human CD14 PerCP− Cyanine 5.5 or isotype matched controls (all from eBioscience, San Diego, CA, USA); anti-human CD4 Alexa Fluor 700, anti-human CD25 PE/Dazzle 594 or isotype matched control (all from BioLegend, San Diego, CA, USA); and anti-human Tim-3 PE or isotype matched control (R&D, Minneapolis, MN, USA). .. Data were obtained from multicolor analysis using a FACS Calibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) and were analyzed using FlowJo software 7.6.2 (Tree Star, Ashland, OR, USA).



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